In sunflower seedlings either regularly watered or subjected to water stress by withholding water for 5 days, plasma membrane was isolated by partitioning in an aqueous polymer two-phase system. In the stressed seedlings an osmotic adjustment of 0.1 MPa took place. Water deficit conditions determined an increase in membrane permeability and in the recovery of plasma membrane proteins. Total and soluble protein levels decreased by 9.4 and 45.8% respectively in the stressed seedlings. The sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the soluble proteins and plama membrane proteins did not show qualitative changes in the protein patterns of both the control and the stressed seedlings. Several quantitative differences in the levels of plasma membrane proteins were observed: the 60, 56 and 37 KDa bands, as well as the 37 to 20.1 KDa group, reached higher levels under stress. These last polypeptides can be identified as peripheral plasma membrane proteins of the bilayer.

Plasma-membrane proteins in water-stressed sunflower seedlings

SGHERRI, CRISTINA;
1992-01-01

Abstract

In sunflower seedlings either regularly watered or subjected to water stress by withholding water for 5 days, plasma membrane was isolated by partitioning in an aqueous polymer two-phase system. In the stressed seedlings an osmotic adjustment of 0.1 MPa took place. Water deficit conditions determined an increase in membrane permeability and in the recovery of plasma membrane proteins. Total and soluble protein levels decreased by 9.4 and 45.8% respectively in the stressed seedlings. The sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the soluble proteins and plama membrane proteins did not show qualitative changes in the protein patterns of both the control and the stressed seedlings. Several quantitative differences in the levels of plasma membrane proteins were observed: the 60, 56 and 37 KDa bands, as well as the 37 to 20.1 KDa group, reached higher levels under stress. These last polypeptides can be identified as peripheral plasma membrane proteins of the bilayer.
1992
Navari Izzo, F.; Sgherri, Cristina; Galleschi, L.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/777139
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