Microbiological valorisation of bio-composites based on polylactic acid and wood fibres

The use of wood fibres for production of bio-based composites has attracted interest in various application sectors ranging from packaging to automotive components and in other high value applications. In the course of the present research activity, several bio-based composites were developed using wood fibres with a compostable polymeric matrix such as polylactic acid (PLA) and a flexible biodegradable polymer such as poly(butylene adipate-co-terephthalate) (PBAT). The developed materials were used for the manufacture of several prototypes for food packaging (trays, boxes for refrigerated or frozen fish, egg box), agricultural applications (pots and yarns), automotive components (spoiler and seats) as well as containers for cosmetics and chemicals. Biodegradability and compostability are desired properties, allowing bio-recycling as end of life scenario, mainly for materials used in food packaging and agricultural applications. Thus, they may be recycled at the end of their life time service producing compost as a value-added by-product. Composting is the main option for bio-recycling but also other valuable pathways can be pursued. Because lignocellulose is one of the components of developed materials, several by-products such as enzymes, reducing sugars, proteins, amino acids, carbohydrates, organic acids, etc. may be obtained from the bio-composites produced. Alternatively, the bio-composites can be also used for the production of yeast biomass. This is important as another recyclability way of the new produced materials. In the present research the bio-composites produced were investigated as substrates for the production of the methylotrophic yeast Pichia pastoris, a potential source of single-cell protein (SCP), β-carotene, and Rhodotorula sp. as potential source of food and feed grade colorant. This is another more valuable alternative to the composting considering also that composting cannot be used to dispose of large quantities of bio-plastics, and in the future it will become more and more important to find alternative routes of valorisation for bio-plastics disposal.