We describe a method to consistently prepare human islets for transplantation. By combining a simple collagenase digestion method and a density gradient purification system, we were able to obtain successful isolations (>/=200,000 islet equivalents, >/=50% purity) in 69% of processed glands. No reagent of animal source was used. Isolated islets were morphologically well maintained and functionally competent, with sterility confirmed in 97% of cases. Two patients were transplanted with islets prepared by this method; graft function was demonstrated for a few months. Improved simplicity and consistency, together with adequate quality of the preparations, are the main features of this isolation method.
|Autori:||Bugliani, M; Lupi, R; Del Guerra, S; Boggi, Ugo; Marselli, L; Sbrana, S; Vistoli, Fabio; Torri, S; Del Chiaro, M; Signori, S; Filipponi, Franco; DEL PRATO, Stefano; Campa, Mario; Corsini, V; Campatelli, A; DI CANDIO, Giulio; Mosca, F; Marchetti, Piero|
|Titolo:||An alternative and simple method to consistently prepare viable isolated human islets for clinical transplantation|
|Anno del prodotto:||2004|
|Digital Object Identifier (DOI):||10.1016/j.transproceed.2004.02.047|
|Appare nelle tipologie:||1.1 Articolo in rivista|