We describe a method to consistently prepare human islets for transplantation. By combining a simple collagenase digestion method and a density gradient purification system, we were able to obtain successful isolations (>/=200,000 islet equivalents, >/=50% purity) in 69% of processed glands. No reagent of animal source was used. Isolated islets were morphologically well maintained and functionally competent, with sterility confirmed in 97% of cases. Two patients were transplanted with islets prepared by this method; graft function was demonstrated for a few months. Improved simplicity and consistency, together with adequate quality of the preparations, are the main features of this isolation method.

An alternative and simple method to consistently prepare viable isolated human islets for clinical transplantation

BOGGI, UGO;Marselli L;VISTOLI, FABIO;FILIPPONI, FRANCO;DEL PRATO, STEFANO;CAMPA, MARIO;DI CANDIO, GIULIO;MARCHETTI, PIERO
2004

Abstract

We describe a method to consistently prepare human islets for transplantation. By combining a simple collagenase digestion method and a density gradient purification system, we were able to obtain successful isolations (>/=200,000 islet equivalents, >/=50% purity) in 69% of processed glands. No reagent of animal source was used. Isolated islets were morphologically well maintained and functionally competent, with sterility confirmed in 97% of cases. Two patients were transplanted with islets prepared by this method; graft function was demonstrated for a few months. Improved simplicity and consistency, together with adequate quality of the preparations, are the main features of this isolation method.
Bugliani, M; Lupi, R; Del Guerra, S; Boggi, Ugo; Marselli, L; Sbrana, S; Vistoli, Fabio; Torri, S; Del Chiaro, M; Signori, S; Filipponi, Franco; DEL PRATO, Stefano; Campa, Mario; Corsini, V; Campatelli, A; DI CANDIO, Giulio; Mosca, F; Marchetti, Piero
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/91056
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