The aim of the present study was to characterize the chemical composition and to evaluate the anti-inflammatory and an- ti-nociceptive properties of the essential oil from the leaves of Phyllanthus muellerianus (Kuntze) Exell. The essential oil was hydrodistilled and characterized by gas chromatography-flame ionization detection and gas chromatography coupled with mass spectrometry analyses. The anti-inflammatory activity was evaluated on carrageenan-induced rat paw edema while the anti-nociceptive test was based on hot plate analysis. The hydrodistillation afforded 0.09% (dry weight basis) of pale yellow oil. Thirty-eight compounds representing 94.8% were identified in the oil. The major components of the oil were hexahydro- farnesyl acetone (11.6%), isocaryophyllene (9.8%), and limonene (9.4%). The oils of P. muellerianus displayed anti-nociceptive effect at a rate independent of reaction time and dose (P < 0.001). The rate of inhibition increased exponentially as the dose increases with optimum activity at 400 mg/kg. The carrageenan-induced edema model revealed the suppression of inflamma- tory mediators at a very high significant value (P < 0.001) for all doses, showing activity comparable to a standard drug at 4 hours after carrageenan injection. Collectively, the essential oils depressed the nociceptors and most likely acted as centrally mediated opioid analgesics while the anti-inflammatory mechanisms of the oil might be related to the decrease in the level of iNOS, and cyclo-oxygenase-2 in the edema paw via the suppression of pro-inflammatory cytokines (TNF-α, IL1-β), NO, and PGE2 production. This study confirms the analgesics and inflammatory activities of P. muellerianus.

Chemical Constituents, Anti-nociceptive and Anti-inflammatory Activities of Essential Oil of Phyllanthus muellerianus

Ascrizzi, Roberta
Penultimo
Investigation
;
Guido, Flamini
Ultimo
Investigation
2019-01-01

Abstract

The aim of the present study was to characterize the chemical composition and to evaluate the anti-inflammatory and an- ti-nociceptive properties of the essential oil from the leaves of Phyllanthus muellerianus (Kuntze) Exell. The essential oil was hydrodistilled and characterized by gas chromatography-flame ionization detection and gas chromatography coupled with mass spectrometry analyses. The anti-inflammatory activity was evaluated on carrageenan-induced rat paw edema while the anti-nociceptive test was based on hot plate analysis. The hydrodistillation afforded 0.09% (dry weight basis) of pale yellow oil. Thirty-eight compounds representing 94.8% were identified in the oil. The major components of the oil were hexahydro- farnesyl acetone (11.6%), isocaryophyllene (9.8%), and limonene (9.4%). The oils of P. muellerianus displayed anti-nociceptive effect at a rate independent of reaction time and dose (P < 0.001). The rate of inhibition increased exponentially as the dose increases with optimum activity at 400 mg/kg. The carrageenan-induced edema model revealed the suppression of inflamma- tory mediators at a very high significant value (P < 0.001) for all doses, showing activity comparable to a standard drug at 4 hours after carrageenan injection. Collectively, the essential oils depressed the nociceptors and most likely acted as centrally mediated opioid analgesics while the anti-inflammatory mechanisms of the oil might be related to the decrease in the level of iNOS, and cyclo-oxygenase-2 in the edema paw via the suppression of pro-inflammatory cytokines (TNF-α, IL1-β), NO, and PGE2 production. This study confirms the analgesics and inflammatory activities of P. muellerianus.
2019
Ogunwande, Isiaka A.; Avoseh, Opeyemi N.; Igile, Daniel O.; Lawal, Oladipupo A.; Ascrizzi, Roberta; Flamini, Guido
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/990030
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