We present a π-conjugated ligand endowed with blue emission and the ability to form a self-assembled red-emitting europium(III) complex with a long emission lifetime (430 μs), even in a complex cellular environment. The ligand is easily synthesized, readily conjugated to antibodies, and suitable for multicolor and time-gated fluorescence microscopy, offering the possibility of imaging cellular structures in distinct spectral and temporal windows.
A Bioconjugable Eu(III)-Based Luminescent Probe for Multicolor and Time-Gated Immunofluorescence
Busato, Francesco;De Felice, Martina;Petri, Filippo;Fausti, Mattia;Bruschi, Blanca;Lenzi, Pietro;Cella Zanacchi, Francesca;Pisignano, Dario;Di Bari, Lorenzo;Arrico, Lorenzo
2026-01-01
Abstract
We present a π-conjugated ligand endowed with blue emission and the ability to form a self-assembled red-emitting europium(III) complex with a long emission lifetime (430 μs), even in a complex cellular environment. The ligand is easily synthesized, readily conjugated to antibodies, and suitable for multicolor and time-gated fluorescence microscopy, offering the possibility of imaging cellular structures in distinct spectral and temporal windows.File in questo prodotto:
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