Solid state detectors made of Si (4.8x8 mm(2)) and GaAs (6.4x8 mm(2)) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with P-32. The system has shown a good minimum detectable activity per unit area of 2. 10(-4) cps mm(-2), and has proved linear for count rate in the range 0.2-20 cpa, typical of autoradiography. The pixel dimensions are 75x500 mu m(2) (Si-Omega2) and 50x500 mu m(2)(GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots).
Use of silicon and GaAs pixel detectors for digital autoradiography
BISOGNI, MARIA GIUSEPPINA;Ciocci MA;DEL GUERRA, ALBERTO;FANTACCI, MARIA EVELINA;ROSSO, VALERIA;STEFANINI, ARNALDO
1997-01-01
Abstract
Solid state detectors made of Si (4.8x8 mm(2)) and GaAs (6.4x8 mm(2)) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with P-32. The system has shown a good minimum detectable activity per unit area of 2. 10(-4) cps mm(-2), and has proved linear for count rate in the range 0.2-20 cpa, typical of autoradiography. The pixel dimensions are 75x500 mu m(2) (Si-Omega2) and 50x500 mu m(2)(GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
