The chloroformic crude extract of roots of Bupleurum fruticosum L. (Umbelliferae) showed a concentration-dependent vasorelaxing effect on aortic rings endothelium-deprived and pre-contracted by norepinephrine (NE). The pharmacological effect was not produced through the stimulation of cyclooxygenase, adenyl cyclase, or guanylyl cyclase, since selective inhibitors did not prevent the extract-induced responses. The incubation of the aortic rings with the chloroformic extract (10−4 g/ml) produced a depression of the concentration–contractile response curve to NE, in normal conditions, and this effect was more evident in Ca2+-free Tyrode solution, suggesting an action on the intracellular mobilization of Ca2+ ions. Moreover, the vasodilator action of Bupleurum fruticosum extract was resistant to the pre-treatment with nifedipine and to the pre-treatment with cyclopiazonic acid (blocker of Ca2+/ATPase). Finally, the chloroformic extract of Bupleurum fruticosum produced a reduction of the contraction obtained by caffeine, an opener of ryanodine-sensitive receptors, suggesting that the plant could elicit the vasorelaxing response by the blockade of ryanodine-sensitive Ca2+ channels of the sarcoplasmic reticulum.

VASORELAXANT EFFECTS OF THE CHLOROFORMIC CRUDE EXTRACT OF BUPLEURUM FRUTICOSUM L. (UMBELLIFERAE) ROOTS ON RAT THORACIC AORTA

TESTAI, LARA;CHERICONI S.;PISTELLI, LUISA;CALDERONE, VINCENZO;
2005

Abstract

The chloroformic crude extract of roots of Bupleurum fruticosum L. (Umbelliferae) showed a concentration-dependent vasorelaxing effect on aortic rings endothelium-deprived and pre-contracted by norepinephrine (NE). The pharmacological effect was not produced through the stimulation of cyclooxygenase, adenyl cyclase, or guanylyl cyclase, since selective inhibitors did not prevent the extract-induced responses. The incubation of the aortic rings with the chloroformic extract (10−4 g/ml) produced a depression of the concentration–contractile response curve to NE, in normal conditions, and this effect was more evident in Ca2+-free Tyrode solution, suggesting an action on the intracellular mobilization of Ca2+ ions. Moreover, the vasodilator action of Bupleurum fruticosum extract was resistant to the pre-treatment with nifedipine and to the pre-treatment with cyclopiazonic acid (blocker of Ca2+/ATPase). Finally, the chloroformic extract of Bupleurum fruticosum produced a reduction of the contraction obtained by caffeine, an opener of ryanodine-sensitive receptors, suggesting that the plant could elicit the vasorelaxing response by the blockade of ryanodine-sensitive Ca2+ channels of the sarcoplasmic reticulum.
Testai, Lara; Chericoni, S.; Bader, A.; Pistelli, Luisa; Calderone, Vincenzo; Martinotti, E.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/181741
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