The seven Anglerfish species, which belong to the genus Lophius, have a different value on the market, worldwide. If whole fishes can be identified by their morphological characteristics, they become indistinguishable when prepared or processed. In this study, a rapid method based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was developed for the authentication of the seven Lophius species, using a cytochrome b gene fragment of 566 bp. After a genus-specific PCR, a fast digestion with the restriction enzyme BfaI, followed by agarose gel electrophoresis, allowed a clear species identification by producing specific restriction patterns. The total time required was as low as 6 h, DNA extraction included. The method was then used to analyse 48 commercial samples, whose phylogenetic analysis confirmed the PCR-RFLP response at 100 %. Results showed that mislabelling occurs on the market regardless the kind of processing.
|Autori:||Armani A; Castigliego L; Tinacci L; Gandini G; Gianfaldoni D; Guidi A|
|Titolo:||A rapid PCR-RFLP method for the identification of Lophius species|
|Anno del prodotto:||2012|
|Digital Object Identifier (DOI):||10.1007/s00217-012-1754-3|
|Appare nelle tipologie:||1.1 Articolo in rivista|