The seven Anglerfish species, which belong to the genus Lophius, have a different value on the market, worldwide. If whole fishes can be identified by their morphological characteristics, they become indistinguishable when prepared or processed. In this study, a rapid method based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was developed for the authentication of the seven Lophius species, using a cytochrome b gene fragment of 566 bp. After a genus-specific PCR, a fast digestion with the restriction enzyme BfaI, followed by agarose gel electrophoresis, allowed a clear species identification by producing specific restriction patterns. The total time required was as low as 6 h, DNA extraction included. The method was then used to analyse 48 commercial samples, whose phylogenetic analysis confirmed the PCR-RFLP response at 100 %. Results showed that mislabelling occurs on the market regardless the kind of processing.

A rapid PCR-RFLP method for the identification of Lophius species

ARMANI, ANDREA;CASTIGLIEGO, LORENZO;TINACCI, LARA;GIANFALDONI, DANIELA;GUIDI, ALESSANDRA
2012

Abstract

The seven Anglerfish species, which belong to the genus Lophius, have a different value on the market, worldwide. If whole fishes can be identified by their morphological characteristics, they become indistinguishable when prepared or processed. In this study, a rapid method based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was developed for the authentication of the seven Lophius species, using a cytochrome b gene fragment of 566 bp. After a genus-specific PCR, a fast digestion with the restriction enzyme BfaI, followed by agarose gel electrophoresis, allowed a clear species identification by producing specific restriction patterns. The total time required was as low as 6 h, DNA extraction included. The method was then used to analyse 48 commercial samples, whose phylogenetic analysis confirmed the PCR-RFLP response at 100 %. Results showed that mislabelling occurs on the market regardless the kind of processing.
Armani, Andrea; Castigliego, Lorenzo; Tinacci, Lara; Gandini, G; Gianfaldoni, Daniela; Guidi, Alessandra
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/199976
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