TheBetaproteobacteria-Euplotesassociationisanobligatorysymbioticsysteminvolvingamonophyleticgroupofciliatespeciesandtwobetaproteobacteriaspecieswhichcanbealternativelypresent.Recentdatashowedthatthisrelationshiphasbeenestablishedmorethanonceandthatseveralsymbiont-substitutioneventstookplace,revealingacomplexandintriguingevolutionarypath.Duetothedifferentevolutionarypathwaysfollowedbythedifferentsymbionts,eachbacterialstraincouldhavedifferentiallyevolvedand/orlostfunctionaltraits.Therefore,weperformedre-infectionexperiments,bothbyphagocytosisandbymicroinjection,totestthepossiblefunctionalroleofthedifferentbacteriatowardstheciliates.Ourresultsconfirmthatthegrowthcapacityofthehostisindissolublylinkedtothepresenceofitsoriginalsymbionts.Resultsoftheattemptsofre-infectionbyphagocytosisshowedthatnoneofthebacteriaisabletosuccessfullycolonizethehostcytoplasminthisway,evenifregularlyingested.Re-infectionbymicroinjectionsucceedonlyinonecase.SuchresultspointtoahighdegreeofspecificityintheinteractionsbetweenbacteriaandEuplotesevenaftertheinvasionstep.Duetoaco-evolutivepathwayofreciprocaladaptation,differentdegreeofre-colonizationabilitycouldhavebeenconservedbythedifferentspeciesandstrainsofthesymbionts.

High degree of specificity in the association between symbiotic betaproteobacteria and the host Euplotes (Ciliophora,Euplotia)

VANNINI, CLAUDIA
Primo
;
PETRONI, GIULIO
Penultimo
;
2017-01-01

Abstract

TheBetaproteobacteria-Euplotesassociationisanobligatorysymbioticsysteminvolvingamonophyleticgroupofciliatespeciesandtwobetaproteobacteriaspecieswhichcanbealternativelypresent.Recentdatashowedthatthisrelationshiphasbeenestablishedmorethanonceandthatseveralsymbiont-substitutioneventstookplace,revealingacomplexandintriguingevolutionarypath.Duetothedifferentevolutionarypathwaysfollowedbythedifferentsymbionts,eachbacterialstraincouldhavedifferentiallyevolvedand/orlostfunctionaltraits.Therefore,weperformedre-infectionexperiments,bothbyphagocytosisandbymicroinjection,totestthepossiblefunctionalroleofthedifferentbacteriatowardstheciliates.Ourresultsconfirmthatthegrowthcapacityofthehostisindissolublylinkedtothepresenceofitsoriginalsymbionts.Resultsoftheattemptsofre-infectionbyphagocytosisshowedthatnoneofthebacteriaisabletosuccessfullycolonizethehostcytoplasminthisway,evenifregularlyingested.Re-infectionbymicroinjectionsucceedonlyinonecase.SuchresultspointtoahighdegreeofspecificityintheinteractionsbetweenbacteriaandEuplotesevenaftertheinvasionstep.Duetoaco-evolutivepathwayofreciprocaladaptation,differentdegreeofre-colonizationabilitycouldhavebeenconservedbythedifferentspeciesandstrainsofthesymbionts.
2017
Vannini, Claudia; Sigona, Cristiana; Hahn, Martin; Petroni, Giulio; Fujishima, Masahiro
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/858417
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