Sixteen lactating Sarda ewes were fed 4 diets differing in the forage/concentrate ratio (2 diets 75/25, 2 diets 60/40, DM) and in soyabean oil supplementation (2 diets with 100 g/head/d oil, 2 diets with no oil), in a 4x4 Latin square design. The inclusion of soyabean oil in the diet resulted in a significant increase of both rumenic acid (cis 9 trans, 11 CLA) and vaccenic acid (trans 11 C18:1) in milk fat, with higher increases with the 2 high forage diets. The 2 low forage diets allowed increases of trans 10 C18:1 and of trans 10, cis 12 CLA, probably due to a shift of rumen biohydrogenation of linoleic acid.
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