Oleocanthal is one of the phenolic compounds of extra virgin olive oil with important anti-inflammatory properties. Although its potential anticancer activity has been reported, only limited evidence has been provided in cutaneous malignant melanoma. The present study is aimed at investigating the selective in vitro antiproliferative activity of oleocanthal against human malignant melanoma cells. Since oleocanthal is not commercially available, it was obtained as a pure standard by direct extraction and purification from extra virgin olive oil. Cell viability experiments carried out by WST-1 assay demonstrated that oleocanthal had a remarkable and selective activity for human melanoma cells versus normal dermal fibroblasts with IC50s in the low micromolar range of concentrations. Such an effect was paralleled by a significant inhibition of ERK1/2 and AKT phosphorylation and downregulation of Bcl-2 expression. These findings may suggest that extra virgin olive oil phenolic extract enriched in oleocanthal deserves further investigation in skin cancer.

Cytotoxic Activity of Oleocanthal Isolated from Virgin Olive Oil on Human Melanoma Cells

FOGLI, STEFANO
Primo
;
ARENA, CHIARA;CARPI, SARA;POLINI, BEATRICE;BERTINI, SIMONE;DIGIACOMO, MARIA;GADO, FRANCESCA;SABA, ALESSANDRO;SACCOMANNI, GIUSEPPE;BRESCHI, MARIA CRISTINA;NIERI, PAOLA;MANERA, CLEMENTINA;MACCHIA, MARCO
Ultimo
2016-01-01

Abstract

Oleocanthal is one of the phenolic compounds of extra virgin olive oil with important anti-inflammatory properties. Although its potential anticancer activity has been reported, only limited evidence has been provided in cutaneous malignant melanoma. The present study is aimed at investigating the selective in vitro antiproliferative activity of oleocanthal against human malignant melanoma cells. Since oleocanthal is not commercially available, it was obtained as a pure standard by direct extraction and purification from extra virgin olive oil. Cell viability experiments carried out by WST-1 assay demonstrated that oleocanthal had a remarkable and selective activity for human melanoma cells versus normal dermal fibroblasts with IC50s in the low micromolar range of concentrations. Such an effect was paralleled by a significant inhibition of ERK1/2 and AKT phosphorylation and downregulation of Bcl-2 expression. These findings may suggest that extra virgin olive oil phenolic extract enriched in oleocanthal deserves further investigation in skin cancer.
Fogli, Stefano; Arena, Chiara; Carpi, Sara; Polini, Beatrice; Bertini, Simone; Digiacomo, Maria; Gado, Francesca; Saba, Alessandro; Saccomanni, Giuseppe; Breschi, MARIA CRISTINA; Nieri, Paola; Manera, Clementina; Macchia, Marco
File in questo prodotto:
File Dimensione Formato  
Cytotoxic Activity of Oleocanthal_2016.pdf

solo utenti autorizzati

Tipologia: Versione finale editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 533.19 kB
Formato Adobe PDF
533.19 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Nutr and Cancer_FINAL_REVISION.pdf

accesso aperto

Tipologia: Documento in Post-print
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 130.45 kB
Formato Adobe PDF
130.45 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/810584
Citazioni
  • ???jsp.display-item.citation.pmc??? 27
  • Scopus 59
  • ???jsp.display-item.citation.isi??? 53
social impact