Ruminants derived products have a prominent role in diets and economy worldwide; therefore, the capability to control the rumen microbial ecosystem, for ameliorating their quality, is of fundamental importance in the livestock sector. e aim of this study was to evaluate the e ect of dietary supplementation with chestnut and quebracho tannins on microbial community and fatty acid pro le, in the rumen uid of dairy ewes. Multivariate analysis of PCR-DGGE pro les of rumen microbial communities showed a correlation among the presence of chestnut or quebracho in the diet, the speci c Butyrivibrio group DGGE pro les, the increase in 18:3 cis9, cis12, and cis15; 18:2 cis9 and cis12; 18:2 cis9 and trans11; 18:2 trans11 and cis15; and 18:1 trans11 content, and the decrease in 18:0 concentration. Phylogenetic analysis of DGGE band sequences revealed the presence of bacteria representatives related to the genera Hungatella, Ruminococcus, and Eubacterium and unclassi ed Lachnospiraceae family members, suggesting that these taxa could be a ected by tannins presence in the diets. e results of this study showed that tannins from chestnut and quebracho can reduce the biohydrogenation of unsaturated fatty acids through changes in rumen microbial communities.

Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes

Cappucci, Alice;Minieri, Sara;Mele, Marcello;
2017

Abstract

Ruminants derived products have a prominent role in diets and economy worldwide; therefore, the capability to control the rumen microbial ecosystem, for ameliorating their quality, is of fundamental importance in the livestock sector. e aim of this study was to evaluate the e ect of dietary supplementation with chestnut and quebracho tannins on microbial community and fatty acid pro le, in the rumen uid of dairy ewes. Multivariate analysis of PCR-DGGE pro les of rumen microbial communities showed a correlation among the presence of chestnut or quebracho in the diet, the speci c Butyrivibrio group DGGE pro les, the increase in 18:3 cis9, cis12, and cis15; 18:2 cis9 and cis12; 18:2 cis9 and trans11; 18:2 trans11 and cis15; and 18:1 trans11 content, and the decrease in 18:0 concentration. Phylogenetic analysis of DGGE band sequences revealed the presence of bacteria representatives related to the genera Hungatella, Ruminococcus, and Eubacterium and unclassi ed Lachnospiraceae family members, suggesting that these taxa could be a ected by tannins presence in the diets. e results of this study showed that tannins from chestnut and quebracho can reduce the biohydrogenation of unsaturated fatty acids through changes in rumen microbial communities.
Buccioni, Arianna; Pallara, Grazia; Pastorelli, Roberta; Bellini, Letizia; Cappucci, Alice; Mannelli, Federica; Minieri, Sara; Roscini, Valentina; Rapaccini, Stefano; Mele, Marcello; Giovannetti, Luciana; Viti, Carlo; Pauselli, Mariano
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/885140
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