Paenibacillus larvae is the pathogen responsible for American foulbrood disease in honeybees. Today, no useful tool has been identified to contrast its spreading due to the spores diffusion, except for hives burning. The effect of glucose oxidase and its reaction products, hydrogen peroxide and gluconic acid, was evaluated against P. larvae ATCC9545. Minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by microdilution method. MIC values were employed to evaluate the survival growth rates of P. larvae after each treatment in a macromethod test. Results showed an inhibitory effect of glucose oxidase, gluconic acid and hydrogen peroxide at a minimum concentration of 0.28 μg/mL, 3.71 μg/mL and 0.74 μg/mL, respectively. For hydrogen peroxide MIC value corresponded to MBC value, while P. larvae survival growth rates at observed MIC was 98.3% and 91.6% for glucose oxidase and gluconic acid, respectively. Glucose oxidase MBC value (74.20 μg/mL) was remarkably higher than its MIC value. Even thought, the present work focused on the in vitro inhibitory effect of glucose oxidase and its reaction products only on P. larvae ATCC9545 vegetative form, the obtained results were promising and repeatable.

Preliminary evaluation of glucose oxidase and its products in vitro antimicrobial activities on Paenibacillus larvae ATCC9545 vegetative form

SAGONA, SIMONA;TURCHI, BARBARA;FRATINI, FILIPPO;TORRACCA, BEATRICE;NUVOLONI, ROBERTA;CERRI, DOMENICO;FELICIOLI, ANTONIO
2015

Abstract

Paenibacillus larvae is the pathogen responsible for American foulbrood disease in honeybees. Today, no useful tool has been identified to contrast its spreading due to the spores diffusion, except for hives burning. The effect of glucose oxidase and its reaction products, hydrogen peroxide and gluconic acid, was evaluated against P. larvae ATCC9545. Minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by microdilution method. MIC values were employed to evaluate the survival growth rates of P. larvae after each treatment in a macromethod test. Results showed an inhibitory effect of glucose oxidase, gluconic acid and hydrogen peroxide at a minimum concentration of 0.28 μg/mL, 3.71 μg/mL and 0.74 μg/mL, respectively. For hydrogen peroxide MIC value corresponded to MBC value, while P. larvae survival growth rates at observed MIC was 98.3% and 91.6% for glucose oxidase and gluconic acid, respectively. Glucose oxidase MBC value (74.20 μg/mL) was remarkably higher than its MIC value. Even thought, the present work focused on the in vitro inhibitory effect of glucose oxidase and its reaction products only on P. larvae ATCC9545 vegetative form, the obtained results were promising and repeatable.
Sagona, Simona; Turchi, Barbara; Fratini, Filippo; Giusti, M; Torracca, Beatrice; Nuvoloni, Roberta; Cerri, Domenico; Felicioli, Antonio
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/765139
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